Spent oil polluted soil is widespread all over Nigeria. The pollution occurs in the urban as well as in the rural areas. This study is aimed at the Bioremediation of spent hydrocarbon-contaminated soil in Apo mechanic village in the federal capital territory Abuja. Standard microbiological method was used in isolation and identification of bacteria from the amended contaminated soil. The total heterotrophic spent hydrocarbon bacteria count in the amended spent hydrocarbon contaminated soil was high. After week 3, the control was 2.88± 0.12×106cfu/g, from chicken dropping recorded 5.81 ± 0.03 x106cfu/g, from cow dung it was 6.91 ± 0.10 x106cfu/g and low in week 1 the control recorded 1.94± 0.21 x106cfu/g, from chicken dropping amended soil 4.13 ±0.90 x106cfu/g, from cow dung it was 5.61 ± 0.25 x106cfu/g. The hydrocarbon-utilizing bacteria isolated were Bacillus sp, Citrobactersp, Micrococcussp, Pseudomonas sp, Flavobacteriumspand Alicagenes sp. Duration effect on hydrocarbon utilization after amending thecontaminated soilwas recorded highest in week 3 the control recorded 3.08± 0.02 mg/g, from chicken dropping it recorded 5.98 ± 0.22 mg/g reduction and from cow dung it recorded 6.01 ± 0.11 mg/g and lowest after week 1 from the control recorded 1.57± 0.21 mg/g, from the chicken dropping it was observed that the reduction was 3.13 ± 0.15 mg/g, from cow dung it was observed that the reduction was 3.81 ± 0.05 mg/g. Physicochemical properties of the control and amended contaminated soil showed the highest in week 3 the pH was 8.6 in chicken dropping and 8.7 cow dung and lowest in week 1 the pH from the control was 6.0, from the chicken dropping it was 7.5, from cow dung it was 7.2. The Total organic carbon was 1.72 in Control. In week 1 the total organic carbon was 2.08 mg/g in chicken dropping and 2.12 mg/g in cow dung and in week 3 it was 3.24mg/g in chicken dropping and 3.45 mg/g in cow dung, it was observed that using chicken dropping and cow dung will help the indigenous bacteria and fungi species in utilization of spent hydrocarbon on contaminated soil.
