This study aimed to evaluate the bacterial divesity, and the antimicrobial potential of Nicotiana tabacum oil extracts against diverse Smokers Mouth Floral isolates The oral microbiota of smokers is significantly impacted by tobacco use, which may increase the prevalence of pathogenic bacteria, potentially leading to oral health complications.. Five (5) oral samples were collected from five smokers, and microbial analysis was carried out using standard microbiological techniques through the use of NA and MacConkey agar. Isolated were identify via cultural characterstics and through series of biochemical reactions. Antimicrobial activities of Nicotiana tabacum oil extracts was performed with varying concertation (100 mg/mL, 50 mg/mL, 25 mg/mL, and 12.5 mg/mL) using agar well diffusion assay. The killing dynamics of Nicotiana tabacum oil extracts against isolated bacteria was done using UV spectrophotometer. Results showed CFUs ranging from 3.0 × 104 to 9.8 × 104 CFU/mL among the samples. This study identified several bacterial isolates from the oral flora of smokers, including Enterococcus faecalis, Staphylococcus aureus, Lactobacillus lactis, Pediococcus acidilactici, Peptostreptococcus anaerobius, Stomatococcus mucilaginosus, Actinomyces bovis, Streptococcus agalactiae, Bifidobacterium bifidum, and Corynebacterium diphtheriae. The oil extracts exhibited significant antibacterial activity, particularly at higher concentrations, with inhibition zones of up to 13.0 mm for Staphylococcus aureus. Killing time assays revealed a reduction in bacterial load, with optical density (OD) dropping to 0.000 by 48 hours for most isolates, demonstrating effective bacterial killing.Nicotiana tabacum oil extracts showed promising antibacterial properties against oral pathogens from smokers. These findings suggest potential applications of the extracts as adjuncts to oral health treatments for smokers, warranting further research into their molecular mechanisms and long-term efficacy.
