Global health is seriously threatened by the rise of multidrug-resistant (MDR) bacteria, which makes the hunt for new natural antimicrobial medicines imperative. The purpose of this study was to assess the potential of the antimicrobial compounds present in the extracts of Night-blooming flower (Nyctanthes arbor-tristis) and Trailing Daisy (Sphagneticola trilobata) that are effective against a variety of multidrug-resistant bacterial strains. Following the extraction of plant materials using solvent-based techniques, using water, DMSO, chloroform and methanol, the disc diffusion method was used to test for antibacterial activity. Multidrug-resistant strains of Staphylococcus aureus, were used to assess the antibacterial effectiveness of the extracts. Significant inhibitory zones were observed in the extracts, suggesting the presence of strong antimicrobial agents. Broad-spectrum action was demonstrated by the antimicrobial compounds; against S. aureus. To evaluate the potency of the active fractions, the minimum inhibitory concentration (MIC) was calculated. These results highlight the potential of Night-Blooming Flower and Trailing Daisy as important sources of bioactive chemicals with antibacterial qualities, providing a substitute for traditional antibiotics in the treatment of diseases triggered by microorganisms that are resistant to several drugs. The development of plant-based antimicrobial therapies requires more research on their pharmacokinetics and toxicity.
