This study investigates the antibacterial abilities of Tamarindus indica, which is a member of the Fabaceae family.The fruit pulp methanol extract, assessing its antibacterial and antifungal activity in comparison to the conventional medications Fluconazole and Ampicillin. The extract exhibited notable antibacterial activity against Staphylococcus aureus, Escherichia coli, Streptococcus pneumoniae, and Pseudomonas aeruginosa, with minimum inhibitory concentration (MIC) values between 0.120 µg/ml and 0.421 µg/ml. The inhibition zones for S. aureus varied from 8 mm at 25 µg/ml to 10 mm at 100 µg/ml, whereas E. coli displayed zones ranging from 8.5 mm to 12 mm, S. pneumoniae exhibited zones from 9.8 mm to 13 mm, and P. aeruginosa showed zones from 9.5 mm to 14 mm. ampicillin, as a reference, exhibited inhibition zones between 16 mm and 25 mm for S. aureus and E. coli, and reaching 26 mm. The extract demonstrated zones of inhibition in antifungal assays against Candida albicans ranging from 13 mm at 25 µg/ml to 20 mm at 100 µg/ml, and against Aspergillus niger from 10 mm to 15 mm, whereas Fluconazole shown superior activity. The findings indicate that the methanol extract of T. indica (Red Tamarind) fruit pulp possesses bioactive components with notable antibacterial activity, presenting potential as a natural medicinal agent, particularly against multidrug-resistant strains. Additional research is required to identify the active ingredients for expanded medicinal applications.
